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Estrogen, in conjunction with growth hormone (GH) and its secretory product insulin-like growth factor 1 (IGF-1), is critical in mediating breast development during puberty, as well as breast maturation during pregnancy in preparation of lactation and breastfeeding.Estrogens are responsible for maturation and maintenance of the vagina and uterus, and are also involved in ovarian function, such as maturation of ovarian follicles.

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Like other steroid hormones, estrogen enters passively into the cell where it binds to and activates the estrogen receptor.In males, estrogen regulates certain functions of the reproductive system important to the maturation of sperm Estrogens are responsible for the development of female secondary sexual characteristics during puberty, including breast development, widening of the hips, and female fat distribution.Conversely, androgens are responsible for pubic and body hair growth, as well as acne and axillary odor.The estrogen: ER complex binds to specific DNA sequences called a hormone response element to activate the transcription of target genes (in a study using an estrogen-dependent breast cancer cell line as model, 89 such genes were identified).Since estrogen enters all cells, its actions are dependent on the presence of the ER in the cell.

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